Survey Two 2001
Prostate
Supplied 34bE12
| A section of prostatic tissue immuno-stained with 34bE12 which is a monoclonal mouse antibody against human high molecular weight cytokeratin. The antibody labels normal squamous, ductal and other complex epithelia. It reacts with benign small-acinous lesions of the prostate. Consistent positive results are found with squamous cell carcinomas, ductal carcinomas, transitional cell carcinomas, thymomas, and epithelioid mesotheliomas. Adenocarcinomas are variably positive. |
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In-house 34bE12
| The section from the same tissue block stained with the in-house 34bE12. There is not significant difference between the supplied and in-house antibodies. The best results for both demonstrations are obtained by peroxidase blocking for 8 min., 2 rounds of microwave irradiation for 13 min. and 8 min., and immuno-stained with 1:30 diluted primary antibody for 45 min. at room temperature. |
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Prostate Specific Antigen
| A
section of prostatic tissue infiltrated by adenocarcinoma, which occupies more than
90% of the tissue area. Immunohistochemically, the tumour cells express prostate specific
antigen (PSA). The PSA demonstration is well performed with good signal to
background ratio; i.e. high target intensity versus clear background. The best
results are obtained by peroxidase blocking for 10 min., pressure cooking for
2.5 min., and immuno-stained with 1:1000 diluted primary antibody for 32 min. at
37 °C.
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Last updated on 31 October, 2001.
Prepared by HKIMLSQAP Anatomical Pathology Panel.
Copyright 2001 HKIMLSQAP. All Rights Reserved.
