Survey Three 2002
|The micrograph is a reactive
lymph node with follicular hyperplasia, sinus histiocytosis and focal increase in vascularity. The
anti-myeloperoxidase antibody may be used to discriminate between lymphoid leukaemias and myeloid
The best results is obtained by peroxidase blocking for 10 min., microwave irradiation for 15 min., and immuno-stained with 1:500 diluted primary antibody for 60 min. at room temperature.
|A section from same tissue block is immunostained with in-house myeloperoxidase antibody. The average result is better than that stained with supplied antibody. The best result is obtained by peroxidase blocking for 10 min., pressure cooking treatment for 2.5 min., and immuno-stained with 1:1500 diluted primary antibody for 32 min. at 37 oC.|
Last updated on 9 November, 2002.
Prepared by HKIMLSQAP Anatomical Pathology Panel.
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